Assessment of immunity against avian colibacillosis induced by an aroA mutant containing increased serum survival gene in broilers

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Assessment of immunity against avian colibacillosis induced by an aroA mutant containing increased serum survival gene in broilers

Colibacillosis is an important disease in the poultry industry which causes serious economic damages. As it is suggested that vaccination is one of the means to control colibacillosis, we tried to investigate the vaccine potential of a ∆aroA derivative of an O78:K80 avian pathogenic Escherichia coli containing increased serum survival gene. 490 chicks were selected as follows: For assessment of...

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aroa gene sequencing of native escherichia coli o78: k80 isolated from avian colibacillosis in iran

aroa is an important gene which produces aromatic amino acids essential for bacterial life. the sequence of this gene was shown to be different in bacteria, so the purpose of this study was sequencing of aroa gene in native e. coli o78: k80 to determine the putative differences between this strain and other e. coli o78: k80 strains in gene bank. pcr was used to amplify aroa gene in native e. co...

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The humoral immunization potential of panax ginseng polysaccharide (GPS) against H9N2 avian influenza virus (H9N2 AIV) in chickens was investigated. The effects of GPS treatment before and during H9N2 AIV infection were determined in chicken embryo fibroblast (CEF) by MTT (3(4, 5-dimethylthiazol-2-yl)-2, 3-diphenyl tetrazolium bromide) assays and quantitative RT-PCR analysis of MHC and cytokine...

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Construction of an iss deleted mutant strain from a native avian pathogenic Escherichia coli O78: K80 and in vitro serum resistance evaluation of mutant

BACKGROUND: Colibacillosis, caused by different serotypes of avian pathogenic Escherichia coli (APEC), is one of the important diseases in poultry industry. The isolate O78 is the most prevalent serotype of APEC in Iran. One of the APEC virulence factors, increased serum survival (iss) gene, is related to serum resistance. The usual form of colibacillosis in avian is extraintestinal, and serum ...

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construction of an iss deleted mutant strain from a native avian pathogenic escherichia coli o78: k80 and in vitro serum resistance evaluation of mutant

background: colibacillosis, caused by different serotypes of avian pathogenic escherichia coli (apec), is one of the important diseases in poultry industry. the isolate o78 is the most prevalent serotype of apec in iran. one of the apec virulence factors, increased serum survival (iss) gene, is related to serum resistance. the usual form of colibacillosis in avian is extraintestinal, and serum ...

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ژورنال

عنوان ژورنال: Brazilian Journal of Microbiology

سال: 2012

ISSN: 1517-8382

DOI: 10.1590/s1517-83822012000100043